Detection of In vitro Antiviral Activity: Antiviral Screening Assays 
Cytotoxicity screen of compounds - We run a basic cytotoxicity assay in order to determine the optimum testing range for your compound of interest. This is done with each virus-specific cell line. Toxicity testing in multiple cell-lines is also available.
CPE-based antiviral screen - A CPE-based assay in 96-well plates will be used to test the compounds antiviral activity. A predetermined concentration range is based on the cytotoxicity assay. This assay is designed to rapidly screen a large number of compounds for their ability to disrupt killing of the cells by viruses of interest.
TCID/Plaque Assays - A virus-specific plaque-based also known as a tissue culture infectious dose (TCID) assay is performed as a secondary assay to the CPE-based 96-well plate assay. This assay is designed to demonstrate a reduction in viral output by the compound of interest.
Virus |
Family |
Details |
|---|---|---|
Dengue |
Flavivirus |
Dengue virus, type 2 |
Respiratory syncytial virus |
Paramyxovirus |
A2 strain |
Yellow Fever |
Flavivirus |
17D vaccine strain |
Influenza |
Orthomyxoviridae |
A/PR/8/34 (H1N1) |
Influenza |
Orthomyxoviridae |
A/Hong Kong/8/68 (H3N2) |
Chikungunya |
Togaviridae (Alphavirus) |
181/25 vaccine strain |
VEE |
Togaviridae (Alphavirus) |
TC-83 vaccine strain |
Junin |
Arenavirus (New World) |
Candid #1 vaccine strain |
Rift Valley Fever Virus |
Bunyavirus |
MP12 vaccine strain |
Vaccinia |
Orthopoxvirus |
NYCBOH vaccine strain |