ETEC Intestinal Colonization
Normal flora is eradicated with streptomycin and cimetidine is used for stomach deacidification. Mice are inoculated with ETEC H10407 by gastric gavage. Colonization is examined at different time points post challenge by measurement of CFUs in the intestine. Bacterial shedding as well as antibody response can be measured in stool samples. Lethality endpoint can also be used using high dose challenge.