IBT Bioservices has established toxic shock models in BALB/c and C57/BL6 for staphylococcal enterotoxins A, B, C, and toxic shock syndrome toxin 1 (TSST-1). The model utilizes LPS as a potentiating agent as described previously by Stiles et al
. Purfied toxins are administered intraperitoneally followed by an injection of LPS at 4 hours post challenge. Mice typically succumb to challenge within 24-72 hours. In addition to morbidity and mortality, cytokine response can be measured at various time points post challenge using a multiplex MSD assay
. In vitro
toxin neutralization assays of sera (using human PBMCs) are also available for vaccine studies.