In Vitro Antibacterial Testing
A key step in drug discovery is screening assays to assess antibacterial activity of compounds. We offer a set of assays in key bacterial families. We can help you select appropriate assays for your compound and mechanism of action. We can also develop custom assays tailored to your specific program. If you don't see what you need or have any other questions, please contact us.
Minimum Inhibitory Concentration (MIC) – MIC is the lowest concentration of a particular antibiotic needed to inhibit visible growth of bacteria. This assay is typically performed on planktonic (free floating) bacterial cells. MIC is determined by broth microdilution followed by inoculation onto agar plates for enumeration. We can also test MIC of antibiotics using disk diffusion assays.
Minimum bactericidal concentration (MBC) - MBC is the lowest concentration of antibiotic required to kill an organism. To determine the MBC, cultures are incubated with serial dilutions of the compound(s) of interest, inoculated onto agar plates and enumerated. A colony count that represents 0.1% (99.9% reduction) of the original inoculum is considered as MBC.
Minimum doubling time/growth curve (MDT) – MDT is the time required for the bacterial population to double. The MDT assay shows the effect of antibiotics on bacterial growth. To determine MDT, bacterial isolates are grown with and without compound in broth culture. Optical density values are recorded at multiple timepoints. MDT is calculated by comparing the optical densities at the timepoints.
|Bacterial species||Number of isolates available||Details|
|Methicillin-Sensitive Staphylococcus aureus||21||Clinical and standard laboratory strains including 1 isogenic pair|
|Methicillin-Resistant Staphylococcus aureus||14||Clinical and standard laboratory strains including 2 isogenic pairs|
|Pseudomonas aeruginosa||12||Clinical strains|
|Acinetobacter baumannii||20||Clinical strains|
|Enterobacter aerogenes||1||Clinical strains|
|Enterobacter cloacae||6||Clinical strains including ESBL, MDR, AMPC|
|Streptococcus pneumoniae||4||Clinical strains|
|Moraxella catarrhalis||3||Clinical strains|