Flaviviruses are a major family of pathogens and include dangerous arthropod-borne diseases such as dengue (DENV), Japanese encephalitis (JEV), yellow fever (YFV), zika (ZIKV) and others.
DENV infects more than 50 million people worldwide every year, causing a range of clinical syndromes from mild febrile illness to Dengue hemorrhagic fever/shock syndrome (DHF/DSS). Development of effective vaccines and therapeutics against DENV is becoming a high priority for governments and the pharmaceutical industry.
Japanese encephalitis causes high fevers and can damage the nervous system. Yellow fever is endemic in tropical regions and has a high mortality rate.
In vitro screens such as cytopathic effect (CPE), neutralization, and yield-reduction assays are used to test the activity of candidate compounds against flaviviruses. Typically Vero cells are used for these cell-based assays. Other cell lines can be evaluated upon request.