Luminex xMAP® Technology
Multiplex assays using the Luminex xMAP® Technology
The Luminex technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere (“bead”) xMAP Technology. This enables a high degree of multiplexing within a single sample volume. The open-architecture of the technology has permitted manufacturers to generate multiplex assay kits for many types of applications. Using the easily configurable xMAP Technology we can develop custom assays that are accurate and cost-effective.
Solution-phase multiplex assays offer the following benefits:
• Reduced sample volume and other redundant consumables
• More data from the same amount of labor
• Faster results due to solution-phase kinetics
The figure below shows the results of an analysis for antibodies to specific bacterial toxins from a single serum sample. Various bacterial toxins were conjugated to color coded microspheres. The serum sample was incubated at various dilutions with beads in a 96-well plate. Beads were washed and then incubated with the detection antibody. Beads were washed and read on the Luminex 200 and the data was analyzed using a 4PL fit.
We are using the Luminex® 200™ analyzer (Luminex and Luminex 200 are registered trademarks of Luminex Corporation).