PCR Assay Services

Real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) assays are highly sensitive, specific, and time-efficient molecular diagnostic methods.  The PCR assay targets a specific region of pathogen’s genomic DNA or RNA, allowing the reliable identification and quantitation of low -level pathogen as well as multiple pathogens from a single sample.  In addition, PCR data analysis utilizes quantitative cycle (Cq) determined by the software package provided with PCR instrument, producing advantages of objectivity of data interpretation and result reproducibility. 

 

Advantages of real-time quantitative PCR (qPCR)

  • Highly sensitive, specific, and rapid molecular quantitative assay
  • High degree of accuracy and reproducibility
  • A high throughput and cost-effective solution
  • A substitute for serology assay in immunocompromised hosts
  • Applicable to a wide range of biological samples and studies.

 

PCR Assay Services

IBT provides both qPCR and RT-qPCR assay services to detect bacterial and viral pathogens in biological samples.  Our PCR assays are optimized and validated based on PCR efficiency, sensitivity, and specificity.  Customizable assays can be developed and validated within four weeks upon client request.  For each assay, we utilize standard operating procedures, quality controls, and well-trained personnel.  Our current PCR assays include:

Viral assays

  • Zika Virus 
  • Dengue Virus serotypes 1-4
  • Influenza Virus Group A
  • Chikungunya Virus 

Bacterial Assays 

  • Mycoplasma spp.