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Influenza Infection Models

Preclinical testing of influenza in animal models provides valuable information on virus-host interactions associated with morbidity and mortality. IBT Bioservices has validated infection models for a variety strains that represent disease progression in humans. These challenge models can be used to evaluate  antiviral drugs to immunomodulators designed to counter the pathology of cytokine storm.

Mouse Influenza Models

The mouse offers a convenient and efficient model for lead identification and preclinical analysis of safety and efficacy. The highly characterized murine system offers a reliable model for for evaluating various immunological parameters to address vaccine design and candidate evaluation. While wild mice are highly resistant, inbred laboratory mouse strains such as BALB/c and C57Bl/6 mice are susuptible to a variety of influenza viruses. Some strains can cause disease in mice without adaptation but for seasonal H1N1 and H3N2 strains adaptation is required. Changes in body weight, health score and mortality are used to evaluate efficacy of treatments and vaccines against specific strains.

Study Endpoints

    • Health score
    • Weight loss
    • Survival
    • Viral load in organs
    • Cytokines and biomarker and HAI readouts

Validation Data:

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Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.

Alpha (UK) – B. 1.1.7 / 501Y.V1

amino acid mutations: del69–70 HV, del144 Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H

Beta (South Africa) – B.1.351

amino acid mutations: K417N, E484K, N501Y, D614G, A701V

Gamma (Brazil) – P.1

amino acid mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I

Epsilon (Ca, USA) B.1.427

amino acid mutations: L452R, D614G

SARS-CoV-2 Parental Strain Wild Type (Wuhan)
SARS-CoV-2 D614G Variant

amino acid mutations: D614G

Epsilon (Ca, USA) B.1.429

amino acid mutations: S13I, W152C, L452R, D614G

SARS-CoV-2 Delta Variant

amino acid mutations: L452R, E484Q

Wishing You a Happy Thanskgiving!

In observance of Thanksgiving our offices will be closed Thursday November 24th through Sunday November 27th. We will resume normal business hours Monday November 28th. Please excuse any delayed responses during this period.

We look forward to assisting you with all your preclinical product and testing needs after the holiday. Happy Thanksgiving!

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