Staph Aureus Sepsis Model

In the BALB/c mouse sepsis model, animals are challenged with an intraperitoneal (IP) injection of S. aureus strain with or without 3% mucin. In the mucin model, mice are challenged via injection with the study specific strain in 3% mucin as previously described (Adhikari, et al, 2012; Fattom, et al, 1996) and even low challenge dose (104-5 CFU/ mouse) lead to death. In the non-mucin model, animals receive challenge inoculum without mucin and a higher challenge dose (107-9 CFU/ mouse) is required to achieve LD90.

In both models the animals are monitored for morbidity and mortality twice a day for 14 days post challenge. The mucin model allows for incremental increases in the challenge dose to differentiate dose response at broader ther- apeutic ranges. We have established LD50 values for various strain using this model; typically, lethality occurs within 24-48 hours post infection.

Staph Sepsis

IBT Bioservices continues to conduct critical work as an essential business under the State of Maryland’s COVID-19 response directive. We have implemented a risk management plan and are continually monitoring the situation to ensure continuation of research according to public health services recommended best-practices for keeping our employees and the community safe. 

The IBT Bioservices team will continue to deliver high quality products and services for every project. If you have any questions contact us at Services@IBTBioservices.com