Toxic Shock Model

IBT Bioservices has established toxic shock models in BALB/c and C57/BL6 for staphylococcal enterotoxins A, B, C, and toxic shock syndrome toxin 1 (TSST-1).  The model utilizes Lipopolysaccharide (LPS) as a potentiating agent as described previously by Stiles et al.  Purfied toxins are administered intraperitoneally followed by an injection of LPS at 4 hours post challenge.  Mice typically succumb to challenge within 24-72 hours.  In addition to morbidity and mortality, cytokine response can be measured at various time points post challenge using a multiplex cytokine assay. For vaccine studies we offer in vitro toxin neutralization evaluation of sera (using human PBMCs).

Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.