Toxic Shock Model

IBT Bioservices has established toxic shock models in BALB/c and C57/BL6 for staphylococcal enterotoxins A, B, C, and toxic shock syndrome toxin 1 (TSST-1).  The model utilizes Lipopolysaccharide (LPS) as a potentiating agent as described previously by Stiles et al.  Purfied toxins are administered intraperitoneally followed by an injection of LPS at 4 hours post challenge.  Mice typically succumb to challenge within 24-72 hours.  In addition to morbidity and mortality, cytokine response can be measured at various time points post challenge using a multiplex MSD assay.  In vitro toxin neutralization assays of sera (using human PBMCs) are also available for vaccine studies.