Alphaviruses In-Vitro

Alphaviruses are arthropod-borne viruses that cause disease in humans and a wide range of domestic and agricultural animals. Interest in the development of alphavirus therapeutics and vaccines has risen in recent years due to epidemics of Chikungunya virus (CHIKV) causing severe illness in Africa and Asia and concerns about possible use of Venezuelan equine encephalitis virus (VEEV) as an agent of bioterrorism.

A major obstacle in the development of antivirals for alphaviruses is the requirement for the work to be performed in a costly high-containment BSL-3 laboratory. IBT Bioservices has established reliable in vitro assays for alphaviruses using attenuated strains of CHIKV and VEEV, allowing testing in a BSL-2 environment.

Our attenuated alphaviruses include CHIKV 181/25, a well-known experimental vaccine strain, and VEEV TC-83, derived from the highly pathogenic strain Trinidad Donkey (TrD).

  • Vero cells infected and CPE detected using a standard crystal violet staining
  • Boxed point is excluded from the curve fitting of CPE assay  due to the interference from cytotoxicity

  • Vero cells infected and cell supernatants titrated using a standard plaque assay

IBT Bioservices continues to conduct critical work as an essential business under the State of Maryland’s COVID-19 response directive. We have implemented a risk management plan and are continually monitoring the situation to ensure continuation of research according to public health services recommended best-practices for keeping our employees and the community safe. 

The IBT Bioservices team will continue to deliver high quality products and services for every project. If you have any questions contact us at