Flaviviruses In Vitro

Flaviviruses are a major family of pathogens and cause dangerous arthropod-borne diseases such as dengue fever (DENV), Japanese encephalitis (JEV), yellow fever (YFV), zika (ZIKV) and others.

DENV infects more than 50 million people worldwide every year, causing a range of clinical syndromes from mild febrile illness to Dengue hemorrhagic fever/shock syndrome (DHF/DSS). Development of effective vaccines and therapeutics against DENV is becoming a high priority for governments and the pharmaceutical industry.

Japanese encephalitis results in high fevers and can damage the nervous system. Yellow fever is endemic in tropical regions and has a high mortality rate.

In vitro screens such as cytopathic effect (CPE), neutralization, and yield-reduction assays are used to test the activity of candidate compounds against flaviviruses. Typically, Vero cells are used for these cell-based assays. Other cell lines can be evaluated upon request.

  • Vero cells infected and viral plaques detected using a standard immunoplaque assay

  • K562 cell infected and percentage of DENV-positive cells analyzed by flow cytometry

IBT Bioservices continues to conduct critical work as an essential business under the State of Maryland’s COVID-19 response directive. We have implemented a risk management plan and are continually monitoring the situation to ensure continuation of research according to public health services recommended best-practices for keeping our employees and the community safe. 

The IBT Bioservices team will continue to deliver high quality products and services for every project. If you have any questions contact us at Services@IBTBioservices.com