Orthomyxoviridae Antiviral Testing Panel

Influenza viruses mutate quickly by antigenic shift and reassortment requiring development and reformulation of new vaccines each year. IBT Bioservices offer in vitro assays to test antiviral resistance development and to evaluate compounds’ ability to inhibit virus-induced activities such as plaque formation (PRNT) or cytopathogenicity (CPE). The table below lists Influenza strains for which we have validated assays (the asterisks indicate strains for which we offer in vivo efficacy testing).

Species/Type
Strains




Infleunza Type A
(H1N1)
A/California/01/2009*
A/Texas/36/1991*
A/Perth/265/2009 (wt.)
A/Perth/261/2009 (antiviral-resistant)*
A/Puerto Rico/8/1934*
A/Mississippi/3/2001
A/Mississippi/3/2001(antiviral-resistant)
A/Texas/48/2009 (antiviral resistant)
A/Louisiana/08/2013 (antiviral resistant)
A/North Carolina/39/2009 (antiviral resistant)


Influenza Type A
(H3N2)
A/Perth/16/2009
A/Philippines/2/1982
A/Hong Kong/1/1968*
A/Victoria/3/1975*
A/Bethesda/956/2006 (antiviral resistant)
A/Texas/12/2007 (antiviral resistant)
A/Washington/01/2007 (antiviral resistant)
Infleunza Type
B/Lee/1940*
B/Brisbane/60/2008

Infleunza Type B
Yamagata Lineage
B/Phuket/3073/2013
B/Utah/09/2014
B/Memphis/20/1996 (antiviral resistant)
B/Florida/4/2006
Influenza Type B
(Victoria Lineage)
B/Washington/02/2019
B/Hong Kong/330/2001
Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.

Alpha (UK) – B. 1.1.7 / 501Y.V1

amino acid mutations: del69–70 HV, del144 Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H

Beta (South Africa) – B.1.351

amino acid mutations: K417N, E484K, N501Y, D614G, A701V

Gamma (Brazil) – P.1

amino acid mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I

Epsilon (Ca, USA) B.1.427

amino acid mutations: L452R, D614G

SARS-CoV-2 Parental Strain Wild Type (Wuhan)
SARS-CoV-2 D614G Variant

amino acid mutations: D614G

Epsilon (Ca, USA) B.1.429

amino acid mutations: S13I, W152C, L452R, D614G

SARS-CoV-2 Delta Variant

amino acid mutations: L452R, E484Q