skip to Main Content
Influenza In Vitro

Influenza viruses (INFV) are segmented RNA viruses that cause seasonal influenza epidemics and influenza pandemics. INFV A infects birds and some mammals (e.g. pigs and humans), while VFV B only infects humans and seals. Both Influenza A and B pose a severe threat to public health and the agricultural economy. Influenza viruses mutate quickly by antigenic drift and reassortment, re- quiring the development and reformulation of new vaccines each year. Influenza strains resistant to almost all known drugs have been identified, making influenza drug development a high priority. Con- cerns about INFV have been further heightened due to the potential for transmission of highly patho- genic avian influenza (HPAI) strains to humans.

MDCK cells infected and CPE detected using a standard crystal violet staining

Vero cells infected and viral plaque detected by a standard immunoplaque assay

INFV plaque formation in PRNT assay.

Back To Top
Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.