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Flow cytometry is a powerful tool for profiling the phenotypic characteristics of millions of individual cells. It replies on the use of fluid systems, fluorescent proteins and laser optical system. The experimental design process includes staining cells with fluorescently labeled antibodies specific to phenotype-defining antigens on the surface or the interior of the target cell. For accurate readings instruments must be calibrated to capture relevant events and exclude coincidental and background signals. Properly stained cells are passaged through a laser beam which will excite the fluorophores conjugated  antibodies bound to the antigen of interest on the cell. Standardised statistical methods then are used to interpret the resulting excitation emission data to gain in-depth details about the  cell characteristics and/or function being analyzed.   

Immunophenotyping

 Differential expression of cell surface markers is key to phenotyping subsets of cells with unique surface profiles. Cell subset identification can be combined with analysis of activation status of markers or makerder exhaustion. We offer a simple panel made up of exclusively cellular surface markers to identify various subsets of immune cells.  This panel can be used to evaluate CD8+ and CD4+ T-Lymphocytes, as well as B-Lymphocytes. Available markers include CD3, CD4, CD8, CD19 and CD20.

T-Helper Cell Panels

T-helper cells are important immune modulators that play a variety of roles to eliminate a range of pathogens. We offer ready-to-use and validated panels to characterize T-helper (TH) cell subsets and to assess T-lymphocytes response to specific antigens. We offer panels for both surface and intracellular marker analysis.

Markers: CD62L, CD8, CD4, Ki-67, RORgt, CD44, FoxP3, Tbet, CD45.

Functional T-cell Panels

Immune checkpoint modulation and adoptive T-cell transfer are key to developing effective immunotherapies.  Flow cytometry-based functional assay are used to characterize the relative abundance of specific immune subsets and to understanding mechanism of action for drugs an therapeutics. At IBT Bioservices we offer a ready-to-use panel to characterize T-helper (TH) cell subsets and to assess the T-lymphocyte response to specific antigens, evaluating both intracellular cytokine response and transcription factors. 

At IBT Bioservices we offer panels validated using mouse, human and non-human primate samples. We can run your animal study and evaluate the samples collected from the animal study using panels customized  to evaluate the specific cell subset of interest for your project. For a free no obligation consultation  call (877) 411-2041 or email us at Services@IBTBioServices.com

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Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.

Alpha (UK) – B. 1.1.7 / 501Y.V1

amino acid mutations: del69–70 HV, del144 Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H

Beta (South Africa) – B.1.351

amino acid mutations: K417N, E484K, N501Y, D614G, A701V

Gamma (Brazil) – P.1

amino acid mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I

Epsilon (Ca, USA) B.1.427

amino acid mutations: L452R, D614G

SARS-CoV-2 Parental Strain Wild Type (Wuhan)
SARS-CoV-2 D614G Variant

amino acid mutations: D614G

Epsilon (Ca, USA) B.1.429

amino acid mutations: S13I, W152C, L452R, D614G

SARS-CoV-2 Delta Variant

amino acid mutations: L452R, E484Q