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Flow cytometry is a powerful tool for profiling the phenotypic characteristics of millions of individual cells. It replies on the use of fluid systems, fluorescent proteins and laser optical system. The experimental design process includes staining cells with fluorescently labeled antibodies specific to phenotype-defining antigens on the surface or the interior of the target cell. For accurate readings instruments must be calibrated to capture relevant events and exclude coincidental and background signals. Properly stained cells are passaged through a laser beam which will excite the fluorophores conjugated  antibodies bound to the antigen of interest on the cell. Standardised statistical methods then are used to interpret the resulting excitation emission data to gain in-depth details about the  cell characteristics and/or function being analyzed.   

Immunophenotyping

Differential expression of cell surface markers is key to phenotyping subsets of cells with unique surface profiles. We offer a simple panel made up of exclusively cellular surface markers to identify various subsets of immune cells.  This panel can be used to evaluate CD8+ and CD4+ T-Lymphocytes, as well as B-Lymphocytes. Available markers include CD3, CD4, CD8, CD19 and CD20.

T-Helper Cell Panels

Immune checkpoint modulation and adoptive T-cell transfer are key to developing effective immunotherapies. Flow cytometry-based functional assay are used to characterize the relative abundance of specific immune subsets and to understanding mechanism of action for drugs. At IBT Bioservices we offer a ready-to-use panel to characterize T-helper (TH) cell subsets and to assess the T-lymphocyte response to specific antigens, evaluating both intracellular cytokine response and transcription factors. 

Markers: CD62L, CD8, CD4, Ki-67, RORgt, CD44, FoxP3, Tbet, CD45.

Functional T-cell Panels

Immune checkpoint modulation and adoptive T-cell transfer are key to developing effective immunotherapies. Flow cytometry-based functional assay are used to characterize the relative abundance of specific immune subsets and to understanding mechanism of action for drugs. At IBT Bioservices we offer a ready-to-use panel to characterize T-helper (TH) cell subsets and to assess the T-lymphocyte response to specific antigens, evaluating both intracellular cytokine response and transcription factors. 

At IBT Bioservices we offer panels validated using mouse, human and non-human primate samples. We can run your animal study and evaluate the samples collected from the animal study using panels customized  to evaluate the specific cell subset of interest for your project. For a free no obligation consultation  call (877) 411-2041 or email us at Services@IBTBioServices.com

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IBT Bioservices continues to conduct critical work as an essential business under the State of Maryland’s COVID-19 response directive. We have implemented a risk management plan and are continually monitoring the situation to ensure continuation of research according to public health services recommended best-practices for keeping our employees and the community safe. 

The IBT Bioservices team will continue to deliver high quality products and services for every project. If you have any questions contact us at Services@IBTBioservices.com