Assay Details

S. aureus secretes a number of virulence factors, among the most prominent of which is the small β-barrel pore-forming toxin α-hemolysin (also known as alpha-toxin).

A rabbit red blood cell lysis assay has been developed to assess the neutralizing ability of antibodies in samples against alpha-toxin. Percentage lysis, percentage neutralization and the NT50 values for the samples (the dilution or concentration of the sample that gives 50% neutralization) can be calculated.

Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.