Assay Details

This assay tests the neutralization capacity of samples against S. aureus bi-component toxins (leukocidins and gamma-hemolysins) in a neutrophil-like induced HL-60 cell line. Leukocidins act synergistically in pairs to induce damage of monocytes, macrophages and neutrophils. Gamma-lysins also act synergistically but affect leukocytes as well as red blood cells.

We can test the neutralization ability of serum samples against the different toxin pairs Panton-Valentine leukocidin (PVL or LukSF), leukocidin ED (LukED), gamma-hemolysin HlgCB and gamma-hemolysin AB (HlgAB).

Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.

Alpha (UK) – B. 1.1.7 / 501Y.V1

amino acid mutations: del69–70 HV, del144 Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H

Beta (South Africa) – B.1.351

amino acid mutations: K417N, E484K, N501Y, D614G, A701V

Gamma (Brazil) – P.1

amino acid mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I

Epsilon (Ca, USA) B.1.427

amino acid mutations: L452R, D614G

SARS-CoV-2 Parental Strain Wild Type (Wuhan)
SARS-CoV-2 D614G Variant

amino acid mutations: D614G

Epsilon (Ca, USA) B.1.429

amino acid mutations: S13I, W152C, L452R, D614G

SARS-CoV-2 Delta Variant

amino acid mutations: L452R, E484Q

Wishing You a Happy Thanskgiving!

In observance of Thanksgiving our offices will be closed Thursday November 24th through Sunday November 27th. We will resume normal business hours Monday November 28th. Please excuse any delayed responses during this period.

We look forward to assisting you with all your preclinical product and testing needs after the holiday. Happy Thanksgiving!

Visit our reagents page 

See our testing service