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PCR Assay Services

Real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) assays are highly sensitive, specific, and time-efficient molecular diagnostic methods.  The PCR assay targets a specific region of pathogen’s genomic DNA or RNA, allowing the reliable identification and quantitation of low -level pathogen as well as multiple pathogens from a single sample.  In addition, PCR data analysis utilizes quantitative cycle (Cq) determined by the software package provided with PCR instrument, producing advantages of objectivity of data interpretation and result reproducibility.

Advantages of real-time quantitative PCR (qPCR)

  • Highly sensitive, specific, and rapid molecular quantitative assay
  • High degree of accuracy and reproducibility
  • A high throughput and cost-effective solution
  • A substitute for serology assay in immunocompromised hosts
  • Applicable to a wide range of biological samples and studies.

PCR Assay Services

IBT provides both qPCR and RT-qPCR assay services to detect bacterial and viral pathogens in biological samples.  Our PCR assays are optimized and validated based on PCR efficiency, sensitivity, and specificity.  Customizable assays can be developed and validated within four weeks upon client request.  For each assay, we utilize standard operating procedures, quality controls, and well-trained personnel.  Our current PCR assays include:

Viral assays

  • Zika Virus
  • Dengue Virus serotypes 1-4
  • Influenza Virus Group A
  • Chikungunya Virus

Bacterial Assays 

  • Mycoplasma spp.
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Fig. 1 Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain). Inoculum 1xLD90=1.0E+05 PFU/mouse
Survival after challenge with INFV H1N1 A/Pert/261/2009 (Tamiflu-resistant strain) 1.0E+05 PFU/mouse
Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu)
Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68.

Alpha (UK) – B. 1.1.7 / 501Y.V1

amino acid mutations: del69–70 HV, del144 Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H

Beta (South Africa) – B.1.351

amino acid mutations: K417N, E484K, N501Y, D614G, A701V

Gamma (Brazil) – P.1

amino acid mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I

Epsilon (Ca, USA) B.1.427

amino acid mutations: L452R, D614G

SARS-CoV-2 Parental Strain Wild Type (Wuhan)
SARS-CoV-2 D614G Variant

amino acid mutations: D614G

Epsilon (Ca, USA) B.1.429

amino acid mutations: S13I, W152C, L452R, D614G

SARS-CoV-2 Delta Variant

amino acid mutations: L452R, E484Q