Flow Cytometry Archives - IBT BioServices

Multiparameter Flow Cytometry Best Practices

Multiparameter flow cytometry is an important tool for biomedical scientists, especially for preclinical drug and vaccine research. It is an invaluable tool for detailed analysis of receptors, signaling, and effector molecules as well as nucleic acids in individual cells and cell populations. Technological advancements have made it possible to design and execute flow cytometry studies that simultaneously evaluate multiple parameters. However, evaluating multiple parameters at the cellular level is not an easy task. Below are some sample preparation, panel design, and data analysis aspects for new and inexperienced flow cytometry users to consider.

Multiparameter Flow Cytometry Notes

One of the most powerful applications of flow cytometry is for evaluating drug and vaccine efficacy. Multiparameter flow cytometry relies on the use of fluid systems, fluorescent proteins, and optical systems to detect and collect fluorophore signals. When the technology first became available multiparameter meant 2 colors, today it can…

Flow Cytometry: A Vital Tool for Evaluating Vaccine Effectiveness

Flow cytometry uses light scattering, light excitation and emission of fluorochrome molecules to gather detailed information about specific cells or particles of interest. Using antibody-fluorophore conjugates flow cytometry allows researchers to draw phenotype-dependent conclusions about cellular phenotype and function. The fluorophores present on bound antibodies excited by lasers yield detailed…