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We are proud to introduce a few of the new products that have recently been added to our product line to support research in filovirus, S. aureus, in addition to other pathogens.

If you have any questions, please contact us.

Recombinant Staphylococcal Enterotoxin K (SEK)

 Catalog #:  0532-001

Description: Recombinant SEK expressed in E. coli and purified to >90% using chromatographic methods. The theoretical molecular weight is 25,326 Daltons.

Size: 100 µg of protein is supplied in sodium phosphate buffer containing sodium chloride and 5% glycerol, at a concentration of 1.45 mg/mL.

Relevance:  SEK is a pyrogenic toxin superantigen secreted by Staphylococcus aureus.  SEK is a powerful activator of T-lymphocytes resulting in substantial cytokine release that can cause severe pathological consequences.

rSEK can be utilized as a control in ELISA and Western blot assays. rSEK can also be used as a potent mitogen, or as an activator of cytokines in Peripheral Blood Mononuclear Cells (PBMC).  

PBMC Stimulation assay: Stimulation of PBMCs using rSEK generates Interferon γ.

S. aureus Hla ELISA Kit

 

Catalog #: 0120-001

Description:  For the quantitative measurement of S. Aureus alpha hemolysin (Hla) in bacterial culture media and serum (mouse and rabbit)

Rabbit anti-EBOV GPΔmuc Polyclonal Antibody

 

Catalog #: 0301-038

Description: Purified rabbit polyclonal antibody reactive to EBOV GP. The antibody detects recombinant EBOV GP (modified) in Western blot and ELISA.

Size: 500 µg of antibody is supplied in DPBS at a concentration of 1.877 mg/mL.

Relevance: The antibody can be used for detection of EBOV GP.

Recommended Dilutions:

  • ELISA: Assay-dependent dilution.
  • WB: Assay-dependent dilution; internal QC demonstrates strong detection of 50 ng of purified recombinant EBOV GP (modified) with a use dilution from 2 µg per mL of antibody in diluent using an HRP antibody conjugate and chromogenic substrate for visualization.

Western blots were detected with anti-EBOV GPΔmuc at 2 µg/mL and visualized using an anti-rabbit HRP conjugate and chromogenic substrate.  Heat-denatured and reduced GP is visualized as multiple bands representing differing glycosylation patterns and GP subunits as observed above: EBOV Makona rGPΔTM (lane 1) and EBOV Mayinga rGPΔmuc (lane 2)

Recombinant Ebola virus Glycoprotein minus the Mucin Domain (EBOV rGPΔmuc – Mayinga)

Catalog #:  0511-025

Description: Recombinant, purified Ebola virus protein with HIS-Tag (EBOV rGPΔmuc – Mayinga strain) is produced in stably-transfected Drosophila Schneider 2 (S2) cells and is purified by FPLC.

Size:  100 µg of protein supplied PBS, pH7.4 (supplemented with glycerol, arginine and glutamic acid) at a concentration of 2.251 mg/mL.

Relevance: Recombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research.

(Panel A) SDS-PAGE demonstrating 1 mg and 5 mg (lane 1, 2 respectively) of EBOV rGPΔmuc protein under denaturing and reducing conditions. MW denotes Novex® Sharp pre-stained protein standard.

(Panel B) Western blot detection of EBOV rGPΔmuc at 50 ng, 100 ng and 500 ng (lanes 1-3) under denaturing and reducing conditions.  EBOV rGPΔmuc was detected using an anti-EBOV rGPΔmuc pAb (cat# 0301-038) at 2.0 mg/mL and anti-rabbit IgG-HRP conjugate, followed by TMB membrane substrate.

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